Microextraction and Determination of Diclofenac in Biological Samples using Hollow Fiber Liquid Phase Microextraction Technique Coupled with HPLC-UV

Document Type: Research Paper

Authors

1 Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch , Islamic Azad University, Tehran, Iran

2 2,4Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

3 Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

Abstract

In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformance
liquid chromatography (HPLC) with UV detection was applied for preconcentration
and determination of Diclofenac sodium in biological fluids. Parameters affecting the extraction
process including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,
extraction time, and salt addition were studied and optimized. Under the optimized conditions
donor phase pH=3, acceptor phase pH=11.7, extraction solvent n-octanol, stirring rate =750 rpm,
extraction time=27 min and without the addition of salt, enrichment factors up to 170 were
achieved and the relative standard deviation (RSD %) of the method was within the range of 2-
3%. The method was successfully applied to determine the concentration of diclofenac sodium in
the biological samples and satisfactory results were obtained.

Keywords