Microwave-assisted Solid-phase(SPPS) and Solution-phase (SPS) Synthesis of Biological Dipeptide ((β-alanine-L histidine)


1 Bionorganic Chemistry Research Laboratory, Chemistry Department, Iran University of Science and Technology, Narmak, Tehran, Iran

2 World Society of the Anti-Aging Medicine and International Hormone Society, Niavaran,Tehran, Iran


Peptides have shown Promising effect as pharmaceutics with the potential to treat a wide
variety of diseases. Peptides are mostly synthesized by biological technology or chemical
methods. Solution phasepeptide synthesis (SPS) and solid phase peptide synthesis (SPPS) are
two major chemical techniques for peptidesproduction.In this research, the synthesis of
dipeptde(β-alanine-L-histidine)wasexaminedby the both SPS and SPPS methods. The
solution phase synthesizeswasdonebyN-phthalic anhydride as a protective group andthe solid
phase using standardtert-Butyloxycarbonyl (BOC) and Fluorenylmethyloxycarbonylchloride
(Fmoc).Inthe both methods,themicrowave was assisted in different steps. The
molecular structure of the dipeptide(β-alanine-L-histidine)  wasdefined using different
spectrometrymethods such as:UV-Vis, FT-IR, 1HNMR and LC-Massanalysis.