Synthesis and Applicationof New Fluorescein Analogues

Document Type: Research Paper



In this project, new derivatives of fluorescein were synthesized using the reaction of maleic anhydride and saccharin with phenol derivatives in the presence of ZnCl2, and their structures were elucidated by UV, IR, and NMR spectroscopy. Fluorimetry studies showed that, the synthesized compounds can be utilized as fluorescent agents, and their efficiencies were dependent on pH of solution. According to the results of HF/6-31+G*//HF/STO-3G calculations as well as experimental studies, the efficiency of fluorescein analogues derived from saccharin was less than the corresponding fluorescein.